![]() The proteins resolve into clearly identifiable sharp bands from 10kDa to 200kDa when analyzed by SDS-PAGE and stained with Coomassie Blue (1). Storage : 4 ☌ for up to 1 year while -20☌ and -80☌ for up to 3 and 10 years, respectively.Protein Ladder, 10-200kDa, is a mixture of 14 recombinant, highly purified proteins designed for precise sizing of proteins by SDS-PAGE. Do NOT heat, dilute, or add reducing agents before loadingĬonstituents : 62.5 mM Tris‐HCl (pH 7.5 at 25☌), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT and 30% (v/v) glycerol After thawing and mixing, load approximately 3-5 µL of protein marker per lane. Usage: The protein ladder comes ready to use in gel loading buffer. We highly recommend to specifically calibrate their MWs by unstained protein MW standard in corresponding systems other than Tris gel/Tris-Glycine buffer. The shifts of the markers in other electrophoresis systems are shown in Table B-D for references. The MWs of them are originally calibrated in Tris gel/Tris-Glycine buffer system (Table A). Therefore, the markers usually show different migration patterns in different electrophoresis systems. The migration of the prestained marker proteins during electrophoresis depends not only on the proteins themselves but also on the coupled dyes. More than half of the bands for Biorad product disappears or becomes very faint. In comparison, Thermo’s 10 KD band is almost completely disappeared, and the two red bands is very faint. After overnight washing, only 25K and 20K of our marker are weakened, and the rest of the bands are still very clear. After 3 hours, all bands of our products are very clear while the brightness of Thermo’s 10 kD band has been extremely weak and Biorad’s 25K band has completely disappeared, and its 10 KD band is also extremely weak. In a controlled experiment, our marker and ones of Thermo and Biorad are subjected to 3 hours to overnight washing with TBST washing buffer. It can take as short as 3 hours or even overnight. The membrane (PVDF or NC) after transferring during western blot experiment needs to undergo blocking, primary and secondary antibody incubation, and a multi-step washing process in between. Note that the red band in Thermo’s product cannot be scanned by NIR fluorescence either. All bands except orange ones are well compatible with near-infrared (NIR) fluorescent system. The images are produced from a single controlled experiment. Dyes only fade slightly and very little or almost no degradation of the marker proteins occurs even under the extreme temperature (37 ☌) ) for one month (lanes 2 and 4). The results for a comparative study under two different temperatures (-20☌ and 37☌) are shown in this image. All the markers are subject to strict stability QC before release. Our Prestained Protein Markers are very stable. Even more, Thermo has many inconsistencies in the molecular weight between its prestained and non-prestained protein markers. Biorad ladder has two inaccurate bands of 37K and 75K while Thermo prestained products have inconsistent 35K and 25K. Biorad and Thermo (Compare lanes 3, 4 and 5 with ours lane 6). MW Precision NewEast Biosciences Prestained Protein Marker is as accurate as the current non-prestained ladders on the market (Compare lanes 1 and 2 with ours lane 6), but has superior MW precision in comparison with the major market suppliers, ie. Highlights – Broad MW range (10-250 kD)Ģ. Ready to use, with no heating, dilution or additional reducing agent required.ġ. The protein markers are highly stable with minimal band broadening during storage. Markers are confirmed in Tris-Glycine SDS-PAGE system with an accuracy of >95% by using unstained protein ladders. ![]() Red bandsĪt 25 kD and 70 kD and provide easy references for molecular weight identification. # : 22201ĭescription: Dual‐Color Prestained Protein Marker – Red Blueĭual-Color Prestained Protein Markers are a mixture of recombinant proteins ranging from 10 kD to 250 kD. Dual‐Color Prestained Protein Marker – Red Blue Cat.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |